I have been doing the isolating also..I hae so many containers markers with an i ..I feel like a nut
I just lost a whole batch of 16 cats to something not sure just yet so I am super parinoid now.
One thing I have noticed if they twitch their heads they sometimes do that to sound or vibration especially the ones closest to pupation. Because mine do that when I speak close to the tent. Even ones in other tents they do the same. I have to remember to be quiet.